By Naoko Yamane-Ohnuki, Kazuya Yamano, Mitsuo Satoh (auth.), Gregory D. Davis, Kevin J. Kayser (eds.)

Great disparities exist among organisms in regards to the relative ease of chromosomal mutagenesis and manipulation. In Chromosomal Mutagenesis, a staff of specialists supply numerous chromosomal manipulation thoughts, together with insertional gene disruptions, gene knockouts, inspired homologous recombination options and different novel instruments, for either prokaryotic and eukaryotic organisms, and try to extend the genetic toolbox past version organisms. Following the structure of the hugely winning Methods in Molecular Biology™ format, every one bankruptcy deals step by step laboratory directions, lists of the required gear and reagents, and tips about troubleshooting and keeping off identified pitfalls.

Comprehensive and state of the art, Chomosomal Mutagenesis covers cutting-edge suggestions which are staged to extend, if now not revolutionize, genetic research within the lengthy ignored and correct phone types.

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